Part:BBa_K3790235:Design

T7 gene product 5.7 controlled by pBAD+dps promoter fusion

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1205
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1144
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 979
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 961
  

Design Notes

The following primers were used for constructing this part.

gp5.7_coding1  	5-GTCCAACTATGTACGGAACAATGCGAGCCTCGTAGCGGAGGCCGCTTCCCGTGGTCAC 	
gp5.7_coding2  	5-CTGCAAGCAATCAAAAGTTGCCCTAAGACTTTCCAGTCCAACTATGTACGGAACAATGC	
gp5.7_coding3  	5-ATGTCTGACTACCTGAAAGTGCTGCAAGCAATCAAAAGTTGCC                	
gp5.7_template1	5-CCCAAGCGCCACCGTTACGTCCgCTAGTAGTCAGGCACGAGATGTGACCACGGGAAGCG	
gp5.7_template2	5-CATTCGTTTCAGAAAGCGAGTACCGGAAGCAGTGATTTCCCAAGCGCCACCG       	
gp5.7_template3	5-TTAGACACATCCTCCCATTCGTTTCAGAAAGCGAGTACC
f_pBAD_dps1	5-CAGCTGTCCCTCCTGTTCAG
f_pBAD_dps2	5-CACCGGCTATGTGTTCCGCTATTCggatccgctaatcttatggataaaaatgc
f_pBAD_GFP	5-ggtgagccagtgtgaATGCATGCGTAAAGGAGAAGAACTTTTCAC
f_pBADtran_tem	5-CAGACATtttttttacctccttaactctagtagctag
r_pBAD_dps1	5-GCGGAACACATAGCCGGTGCTATACTTAATCTCcatacccgtttttttgggctagctac
r_pBAD_dps2	5-CTGAACAGGAGGGACAGCTG
r_pBAD_GFP	5-ggaggtaaaaaaaATGTCTGTTATTTGTATAGTTCATCCATGCCATGTGT
r_pBADtran_tem	5-GCATtcacactggctcaccttc